Laboratory 4

Cell Culture Virology & Research

Laboratory 4 has been partitioned into two sections for the following activities:

Cell culture operations

Preparation of ultrapure reagents used for PCR, and other cleanroom procedures .

Molecular biology

The clean-room section of Lab 4 is equipped with a laminar flow cabinet with HEPA filtration, a Zeiss Invertoskop for examination of uninfected cell culture lines, multiple CO2 incubators and a laser particle counter for continuous monitoring of indoor air quality. Activities that must be conducted in the absence of infectious microbes are performed in this room, with refrigerator and freezer space dedicated exclusively towards storage of noninfectious materials.
Section of molecular biology includes 2 thermocyclers, 1 real-time thermocycler, gel electrophoresis, gel documentation system, and hybridization. This lab provides necessary equipment to perform PCR, RT-PCR, qPCR, and genotyping.