Aerobic Spores are bacteria that form an encapsulated spore as a survival mechanism in response to changes in environment and food availability. They become dormant until more optimum conditions come along and allow them to flourish again.
Aerobic spores are ubiquitous in the environment, present in water, soil, and air. They can be found on vegetation, in dust, and even in intestinal tracts. Many types of bacteria produce spores, including both pathogenic and non-pathogenic varieties.
Aerobic Spore Count is used to assess the removal of particulates from water by different physical removal techniques. This assay is frequently used to determine the effectiveness of filtration systems for particle removal.
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Samples are pasteurized to inactivate interfering vegetative bacteria, while preserving any spore-forming bacteria that would survive harsh conditions. The samples are then filtered through a 0.45 micron membrane that is transferred to selective media and incubated overnight. Bacterial colonies are then counted to determine the number of spores present.
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